LSM is intended for use in experimental sulfur based nitrate reduction equipment or systems. Nitrate an pH test kits are required to fine tune any nitrate reducing equipment. Sulfur based nitrate reducing bacteria require an oxygen depleted environment. Be aware that this process may strongly acidify surrounding water. A typical system employs two separate canisters with a means to control water flow rate through the reactor. The first canister contains the LSM. The second canister is filled with aragonite to neutralize acidified water and supply calcium ions as a by-product of neutralization. LSM can be place in any oxygen restricted environment.